Journal:

Article Title: NALP1 is a transcriptional target for cAMP-response-element-binding protein (CREB) in myeloid leukaemia cells

doi: 10.1042/BJ20040867

Figure Lengend Snippet: (A) Partial sequence of the NALP1 promoter. Putative transcription factor-binding sites are underlined. (B) Control (Cont; untreated) or PMA-treated TF1 cells were analysed for the formation of CREB–DNA complexes by EMSA using a radiolabelled probe from the NALP1 promoter. Nuclear extracts were pre-incubated with antibodies (α-) specific for CREB, phospho-CREB (pCREB) or ATF1, or with 100-fold molar excess of unlabelled CREB-specific probe (+100xSP). (C) Cells were treated with 10 ng/ml PMA for 24 h and then total RNA was extracted and analysed for the expression of NALP1 by real-time PCR. The histogram shows the means±S.D. for triplicate analyses.

Article Snippet: Supershifts were performed using antibodies specifically against CREB, ATF1 (activating transcription factor 1) (both from Santa Cruz Biotechnology, Santa Cruz, CA, U.S.A.) and phospho-CREB (Upstate).

Techniques: Sequencing, Binding Assay, Incubation, Expressing, Real-time Polymerase Chain Reaction